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ESIBIO/BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL/100 µL/ST11006

  • 产品编号: ST11006
  • 美  元  价: $270.00
  • 市  场  价: ¥0.00
  • 品       牌:
  • 产       地: 美国(厂家直采)
  • 公       司:
  • 产品分类: 显微镜>影像显示>仪器类>
  • 公司分类: Antibodies
ESIBIO/BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL/100 µL/ST11006
  • ESIBIO/BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL/100 µL/ST11006
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Description

Details

BioLite™ surface markers allow for direct immunofluorescent staining of live, unfixed pluripotent stem cell populations. The antibodies undergo meticulous application testing and validation to ensure they are sterile, non-toxic, contain no sodium azide, and are free of culture pathogens. Colonies stained with BioLite antibodies are able to continue in culture with no adverse effect on proliferation and differentiation potential when compared to untreated cells.Background Stage-specific embryonic antigen-1 (SSEA-1) is a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma (EC), murine embryonic stem (ES), and murine and human germ (EG) cells. No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.

Details

Details

Concentration0.5 mg/mL
Species ReactivityHuman, Mouse
HostMouse Monoclonal
CloneMC-480
IsotypeIgM, κ
ImmunogenF9 tetracarcinoma stem cells (X-irradiated)
Formulation0.2 μm filter sterilized phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 eu/μg="" of="" the="" protein=""><0.01 ng/μg="" of="" the="" protein)="" as="" determined="" by="" the="" lal="" test.="" formulation="" is="" free="" from="" bacteria,="" fungi,="" and="">
Storage and StabilityStore at 2-8°C protected from light. Stable for 6 months from date of receipt when stored as directed. The BioLite format contains no preservative and therefore must be handled under aseptic conditions.
Applications Tested Immunofluorescence (IF) on live, unfixed mouse ES cells
Recommended DilutionsImmunofluorescence 1:100 Flow Cytometry 1:100 It is recommended that the antibody be titrated for optimal performance for each application.
Alternative NamesCD15, Stage-specific embryonic antigen 1
ReferencesBrambrink, T., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159. PMID: 18371436Draper, J.S., et al. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258. PMID: 12033729
Technical Documents

ST11006 Technical Data Sheet Protocol: Immunofluorescent Staining of Live Cells

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ESIBIO人类胚胎干细胞(hESC)系是根据当前的良好生产规范(cGMP)条件在人类成纤维细胞饲养层上衍生的,可用于研究和临床级格式。作为最早的临床级hESC系,ESI细胞系是当今可用的最佳特征和有据可查的,具有完整的基因组序列,STR指纹图谱和HLA型数据。ESI细胞系的

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  • 完整的基因组概况和全面的数据可供购买

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