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ESIBIO/SSEA-4 anti-Human Antibody, 100 µL/100 µL/ST11015

  • 产品编号: ST11015
  • 美  元  价: $164.00
  • 市  场  价: ¥0.00
  • 品       牌:
  • 产       地: 美国(厂家直采)
  • 公       司:
  • 产品分类: 转染试剂>基因编辑>分子类>
  • 公司分类: Antibodies
ESIBIO/SSEA-4 anti-Human Antibody, 100 µL/100 µL/ST11015
  • ESIBIO/SSEA-4 anti-Human Antibody, 100 µL/100 µL/ST11015
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Description

Details

This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4), a glycolipid carbohydrate epitope that is expressed upon the surface of human teratocarcinoma stem (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. No immunoreactivity is evident with undifferentiated murine EC, ES/iPS and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES/iPS cells may be accompanied by an increase in SSEA-4 expression.

Sample Data

antibody st11015

Figure A: FC analysis on H1 human ES cells at a 1:100 dilution.Red histogram represents SSEA-4 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgG was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with SSEA-4 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).

Details

Details

Concentration0.5 mg/mL
Species ReactivityHuman
HostMouse Monoclonal
CloneMC-831-70
IsotypeIgG3
ImmunogenHuman embryonal carcinoma cell line 2102Ep
FormulationAqueous buffer, 0.09% sodium azide.
Storage and StabilityStore at 2-8°C. Stable for 6 months from date of receipt when stored as directed.
Applications Tested Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF)
Recommended DilutionsFlow Cytometry 1:100Immunocytochemistry/Immunofluorescence 1:100It is recommended that the antibody be titrated for optimal performance for each application.
Alternative NamesStage-specific embryonic antigen 4
ReferencesBattula, V.L., et al. (2007) Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation. PMID: 17288545Kannagi, R., et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J 2: 2355-2361. PMID: 6141938
Technical Documents ST11015 Technical Data Sheet

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  • ICC (5)
  • flow (5)
  • hES (14)
  • human embryonic stem cell (4)
  • SSEA4 (1)
  • hiPS (8)

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ESIBIO的PureStem细胞系是人类胚胎干细胞来源的克隆胚胎祖细胞。因此,它们显示出基因表达的再生(胎儿前)模式,例如缺少COX7A1PureStem祖细胞具有独特且在表型上不同的胚胎祖细胞,它们代表早期胚层,包括外胚层,中胚层,神经C和内胚层。在PureStem分化试剂盒中培养时,祖细胞会表达功能或基因表达差异的表型。包括间质上皮的肌源性和神经源性表型。许多PureStem祖细胞通过遵循已知的细胞命运(包括软骨,成骨,内皮和上皮细胞命运)来生成独特的分化细胞和组织。PureStem祖细胞具有克隆纯净性,并具有未分化和分化命运的基因表达谱。它们不表达端粒酶,

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